About Me |
---|
I am an experienced Bio-Medical Science Professional with 16 years of experience and strong academic background, having completed Masters and Doctorate in Biotechnology. My areas of interests include Microbiology, Immunology and Industrial Biotechnology. I worked as Assistant Professor / HOD – Biotechnology, Department of Biotechnology, Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa) India and acting as Visiting Faculty for the Dept. of Biotechnology, Berhampur University, Orissa. I also possess research exposure of 4.11 years including doctoral degree tenure. Presently I am working as Professor and Head Dept.of Plant pathology MSSSoA,Centurion University of Technology & management since 2016. Professional experience: Teaching : 16 Years 5 months (As on 8/05/2020) Designing of New curricula: Contributors in designing Environmental Biotechnology curriculum ( M.Sc. Biotechnology 2012-14) ,Berhampur university . Mentoring Mentor for post graduate students of M.Sc. Biotechnology ,B. Pharm & Ag. B.Sc. Computational skills: MS-office, Retrieval of Biological databases Major courses studied and taught: Biochemistry, Immunology, Microbiology, Molecular Biology, Bio instrumentation, Cell Biology, Industrial Biotechnology, Animal biotechnology. Major technical skills: Biochemistry Spectrophotometry, HPLC, GC, Centrifugation methods. Molecular Biology DNA isolation from bacteria, blood, Plasmid isolation, Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis, Cloning, RNA isolation,PCR. Protein Science Protein extraction and quantification, Polyacrylamide Gel Electrophoresis (native and SDS PAGE). Bioinformatics BLAST analysis of sequences |
Research expertise
Sl. No. | Title | Issuer |
---|
Submitted as Co-Author:
Abstract: Cancer is disease caused by alterations of the genome as well as the proteome. These changes allow the
cancer cell to evade normal cellular control mechanisms and to start growing uncontrolled. One of the
fundamental features of cancer is tumor clonality, the development of tumors from single cells that begin
to proliferate abnormally. The major goal of cancer research is to identify these molecular defects and use
this knowledge to develop effective diagnostic, treatment and prevention regimens. The generalized loss
of growth control exhibited by cancer cells is the net result of accumulated abnormalities is multiple cell
regulatory systems and is reflected in several aspects of cell behavior. Plumbagozeylanica, commonly
known as ceylon Leadwort or Doctrobush, is a species of plumbago is known to have anticancer effect. It
causes pharmacological importance in medicine and employed clinically fo their antifertility, germicidal,
antileportic and anti-inflammatory activities. Besides it possess central nervous system stimulatory,
hepatoprotective, antioxidant, hypolipidaecmic and anti-atherosclerotic properties. The root is used as
laxative, expectorant, astringent, abortifacient, and in dysentery, the leaves are used as aphrodisiac and
in scabies. Plumbagin(5-hydroxy-2methyl-1, 4-napththoquinone) is a naphtoquinone and as the active
ingredient Responsible for therapeutic effects. It exhibits effective cell growth inhibition by inducing
cancer cells to undergo G2/M phase arrest and apoptosis.
ABSTRACT
Abstract:Marine microorganisms continue to be a rich repository of structural and biologically active novel compounds with potential use in the Pharmabiotech industry. The unique physiochemical properties of
the marine environment (such as pH, pressure, temperature, osmolarity) and uncommon functional
groups (such as isonitrile, dichloroimine, isocyanate, and halogenated functional groups) are frequently
found in marine metabolites. These facts have resulted in the production of bioactive substances with
different properties than those found in terrestrial habitats. In fact, the marine environment contains a
relatively untapped reservoir of bioactivity. Recent advances in genomics, metagenomics, proteomics,
combinatorial biosynthesis, synthetic biology, screening methods, expression systems, bioinformatics,
and the ever increasing availability of sequenced genomes provides us with more opportunities than
ever in the discovery of novel bioactive compounds and biocatalysts. The combination of these advanced
techniques with traditional techniques, together with the use of dereplication strategies to eliminate
known compounds, provides a powerful tool in the discovery of novel marine bioactive compounds.
This review outlines and discusses the emerging strategies of these bioactive compounds
Keywords: Biocatalyst discovery, Dereplication, Marine bioactive compounds, Metaproteomic, Omic
approaches, Synthetic biology.
Abstract: Mycotoxins are secondary metabolites produced by toxigenic species of fungi, which can cause toxic
effects in humans and animals. Food contamination by mycotoxins has been reported worldwide, mostly
in foods that are susceptible to fungal growth, such as grains and cereals. Mycotoxins can lead to damage
to health, and economic losses in agriculture. The most common mycotoxins found in routine food
consumption and with consumption limits set by many countries, including Brazil, are: aflatoxins B1, B2,
G1 e G2, ochratoxin A, zearalenone, deoxynivalenol, T-2 toxin and fumonisin.The contamination of foods
and feeds with Mycotoxins can have serious consequences for human and animal health. Mycotoxin
exposure is most likely to occur in the developing countries where food handling and storage processes
are suboptimal, malnutrition is common, and where handful of regulations exist to protect the exposed
populations. The better understanding of the biochemistry and metabolism of mycotoxins is necessary to
avoid problems caused by them and minimize their negative effects on agriculture and health.
Abstract: Microbial flora can be a source of numerous allergic and infective illnesses. Atmosphere is a hostile environment for micro-organisms, this information provides indoor air quality and on associated potential risks in hospitals. Samples were collected from 20 wards by settling plate technique during January-march 2012 in Government Hospital, Pathapatnam, Andhra Pradesh, INDIA. The number of colonies per plate was variable (15-255 colonies). Maximum number of colonies were observed, the organisms isolated during this study were micrococci, aerobic spore bearing bacilli (asb) coagulase negative staphylococci, gram negative bacilli. Micrococci were isolated from 15 wards, ASB from 12 wards, coagulase negative staphylococci from 12 wards, gram Negative bacilli in 2 wards. Antibiotic sensitivity testing was done by Kirby Bauer’s method for coagulase negative staphylococci. They were found sensitive to Gentamicin, Kefbactam, ciprofloxacin, ofloxacin, and cloxacillin in descending order of sensitivity.
Keywords
Antibiotic sensitivity Test, Indoor air quality, Kirby Bauer’s method, coagulase negative staphylococci, aerobic spore bearing bacilli, Settling plate technique
Abstract: Cyanobacteria can form dense eutrophic and harmful algal blooms (HAB). It can contribute towards
atmospheric nitrogen fixation and influence the global nutrient cycles. Hence the study of their
physiology, biochemistry and genetics is of much importance since last few decades. A cyanobacterial
population provides an excellent model to identify the underlying molecular mechanisms which allow
their success in water columns with higher intensities of light and extreme levels of nutrients. As
cyanobacteria are primitive organisms, they provide novel insights into the genetic adaptations to their
environmental changes happening due to global warming, high Photosynthetically Active Radiation
(PAR) and UV-Radiation along with anthropogenic impacts. Comparing different types of species to
different conditions enables better understanding of different physiological pathways linked to toxin
production and bloom formations. The complete analysis of cyanotoxin biosynthesis-gene clusters (eg.
Nodularin, Anatoxin, Microsporin, Cylindrospermopsin etc.) and the developments of their genetic
manipulation techniques are of high importance in this area of research. However, toxin production
depends on biological, physical and chemical factors encountered in the ecosystem. Genetic markers
rather than traditional methods, presents a tremendous way to understand the interrelations, allelopathic influences and these are critical to understand the hidden secrets. In geographical distribution of
cyanobacterial species, there is large information still to be explored to reveal the phylogenic findings. In
this review, few attempts were made to show possible solutions towards the above mentioned problems
and are discussed in a general way emphasizing on genetic markers along with few other models.
Keywords: Cyanobacteria, genetic markers, PAR, UVR, algal blooms
Submitted as Co author :
Abstract: Cyanobacteria belong to an ancient group of photosynthetic prokaryotes with pronounced variations in their cellular
differentiation strategies, physiological capacities and choice of habitat [9]. The availability of complete genome
sequences of some of the cyanobacteria occupying remarkably diverse ecological niches enabled to gain insights into
their adaptive properties. The objective of this study was to analyze complete cobalt, nickel, zinc, cadmium, Mg+2,
Mn+2, molybdate, chromate ion metal transporters (metal transportomes) from eleven representative Cyanobacterial
species and to identify metal transporters which is associated with "Genomic Island".
Submitted as co author:
Abstract:The content of bacterial cells (cytoplasm) is surrounded by a lipid bilayer, called the cell membrane that
protects the cell from outside influences and makes it possible to carefully control the composition of the
cytoplasm. In Gram-positive bacteria the cytoplasm is bordered by a single phospholipid bilayer, the cell
membrane, and a peptidoglycan layer, the cell wall, which supports firmness of the cell. Gram-negative
bacteria possess an additional outer membrane creating a periplasmic space. The outer membrane of
Gram-negatives is permeable, allowing small molecules to enter the periplasm. In contrast, the
cytoplasmic membrane forms a selective barrier especially for bulky hydrophilic compounds that cannot
easily pass the hydrophobic membrane. To get trace metals, nutrients into and breakdown products out
of the cell, membrane embedded proteins, called transport proteins, mediate the translocation of trace
metals, solutes across the cell membrane. Most transport proteins are very selective and only carry one or
a few related compounds (metals, nutrients) from one side to the other. Not all metal or nutrient
transport systems need to be present at the same time, since usually limited numbers of different
essential metals are present in particular habitats or growth media.
Keywors: metals, nutrients, products, numbers, cytoplasm.
ISBN : 978-93-94457-81-2
ISBN (10): 1-5275-8902-1,ISBN (13): 978-1-5275-8902-5
1. Panda. A, Praveen B & Bisht .S.P.S (2009) “Clinical and pathological Status of haemoglobinopathies among Pregnant Women in Southern Orissa”, Indian Journal ofBiotechnology.Vol-8,pp 456-457. ISSN:0972-5849.
2. S. P. S. Bisht,B. Praveen, Y.Rajesh and Subhasish Sahoo (2010).Bioconversion of Sludge from integrated paper and pulp industries, International J Pharmaand Bio SciencesVol-1(2). ISSN: 0975-6299.
3. B.Praveen,Y.Rajesh,V.Rajakumar,ManasBoxi&K.Mangamma (2010).Extraction, Phyto- chemical Screening and In-vitro evaluation of anti oxidant properties of Commicarpus chinensis (aqueous leaf extract) International J Pharma and Bio Sciences,Vol.1/Issue-4/2010. ISSN: 0975-6299.
4. Panda A.K., Bisht .S.P.S, B. Praveen (2011) “Isolation and 16SrRNA sequencing ofPorphyrobacter sp. AK-P3, an aerobic bacteriochlorophyll-containing sulphur reducing bacterium from an Indian hot spring”, InternationalJournal of Contemporary Research and Review. Vol. 2 (I)1-5. ISSN: 0976 – 4852.
5. Praveen.B, Panda A.K and Bisht, S.P.S (2011) Isolation and characterizationof antibioticproducing bacteria Pseudomonas sp.BP-2 from Nagavali river basin of Srikakulam, Andhra Pradesh, India. Journal of Advanced Biotechnology- Vol.2 Issue 12(June 2011) ISSN:0973-0109.
6. Satpal Singh Bisht, Jayant Mandal, B. Praveen and Amrita Kumari Panda (2011) Investigations on gastro protective effect of rabbitanti- rat gastrin antibody in wister albino rat.Journal of Environment andBioSciences.,2011:Vol.25(1):77-79ISSN: 00973-6913.
7. Satpal Singh Bisht, B.Praveen, Amrita Panda, SantoshBehera (2011).Comparitive genomicsof beta subunit of Tryptophan synthase among a group of proteobacteria. International JPharma and Bio Sciences.Vol-2(4),Oct-Dec2011. ISSN: 0975-6299.
8. Satpal Singh Bisht,B.Praveen, Amrita Panda, and V.Rajakumar (2011) Isolation, Purificationand Characterization of Bacitracin from Bacillus sp. International Journal of Pharmacy andPharmaceutical Sciences.Vol 3 Issue 4, 2011. ISSN:0975 – 1491.
9. S.P.S. Bisht, B. Praveen, Amrita Panda and Sisir Das (2011) Preliminary screening ofSubsurface soils from Radionuclides and Metal contaminated area of Orissa Coast. International J Pharma and Bio Sciences.Vol.2,Issue 3,July-Sep 2011.ISSN 0975-6299.
10. Satpal Singh Bisht, B.Praveen, AmritaPanda, R.Sarita SantoshBehera,& Koustava Kumar Panda,(2011) Isolation, Purification & Characterization of Asparginase from E.coli .Int.J Pharma and BioSciences.Vol-2(4),Oct-Dec2011. ISSN 0975-6299.
11. B.Praveen, Amrita Panda, Sisir Das and S.P.S.Bisht (2011) Isolation of microbes from Radionuclides and Metal contaminated sub-surface soils for Bioremediation of Radioactivewaste management. Asian journal of environmental Sciences June-2011,Vol.6(1):53-57. ISSN: 0973-4759.
12. SatpalS Bisht, K.Raju Patro, B.Praveen*, Amrita Panda, Kaustav Panda (2011).Isolation, Purification & Characterization of Acidolin from Lactobacillus sp. Journal of Environmentand Bioscience.2011:Vol.25(2):235-239ISSN: 00973-6913.
13. Satpal Singh Bisht, RojitaMishra, Koustava K Panda, Amrita Kumari Panda, B .Praveen andAjit Routray (2011) Phytoremediationstudies on coal mine wasteand coal fly ash by Leucaena leucocephala.International Journal of Bioscience, Biochemistry and Bioinformatics. 2011Vol.1(4): 252-255 ISSN: 2010-3638.
14. Satpal Singh Bisht, B.Praveen, Amrita Panda, Santosh Behera, Koustava Kumar Panda,Rojita Mishra, SubhraKanta Patro (2011)Comparative study of various Mycotoxins againstfew bacterial test organisms. International Journal of Pharmacy and Pharmaceutical Sciences.2011.Vol.3(5):288-291.ISSN-0975- 1491.
15. SatpalSingh Bisht,B.Praveen and Rukmini (2011) Screening of paper industry effluent forheavy metal absorbing bacteria. Int.J Pharma and Bio Sciences. Vol3(1);77-81.ISSN 0975-6299.
16. Satpal Singh Bisht, Ravikumar B.V.V, B.Praveen, Rajakumar.V and S. R. Mishra (2011)Effect of Dichlorvoson serum enzymesof poultry birds. Int.J Pharmaand Bio Sciences. Vol 3/Issue 1/Jan – Mar 2012, ISSN 0975-6299.
17. Satpal Singh Bisht, Ravi kumar B.V.V, B.Praveen and S. K. Mishra (2011)Effect of Cypermethrin on serum enzymes of poultry birds. Journal of Pharmaceutical and Biomedical sciences,2011,12(18).ISSN:2230-7885.
18. Satpal SinghBisht,B.Praveen,Amrita Panda, Rojita Mishra, and Harman Preet Kaur Dhillon (2011) Isolation,purificationanddetection of antimicrobial activity of betalactam antibiotics from fewfungal strains.International Journal Pharmaceutical Sciences Review andResearh, Volume 12, Issue 1, January – February2012; ISSN: 0976-044x.
19.SatpalSinghBisht,B.Praveen,AmritaPanda,Rojita Mishra, and Triptisahu(2011).Evaluation of indicator and antioxidant activityof hybrid rose Rosa hybrida. Journal ofPharmacy Research. Jan 2012,Vol.5,Issue 1 ,428-429.ISSN No: 0974-6943.
20.Satpal SinghBisht,B.Praveen ,Rukmini andHarman Dhillon (2012) Isolationand screening of heavy metal absorbingbacteria from theindustry effluent sites of the riverNagavali .International Journalof Pharmaceutical sciences and research.2012; Vol. 3(5):1448-1451.ISSN No:0975-8232.
21.Praveen.B and Bisht, S.P.S (2011) Molecular characterization ofantibiotic producingbacteria Pseudomonas sp.BP-1 from Nagavali riverbasin of Srikakulam, Andhrapradesh, India,Current Trends inBiotechnology and Pharmacy- ISSN No. 0973-8916.Vol-6(1):79-83.
22. Satpal Singh Bisht,B.Praveen ,AmritaPanda,Rojita Mishra and Ramani Priya(2012) Antimicrobial properties of few plants used in traditional system of medication International Journal of Research in Ayurveda and Pharmacy. Vol 3, Issue1, Jan-Feb 2012ISSN No:2229-3566.
23..Satpal Singh Bisht,B.Praveen,Amrita Panda, Rojita Mishra, Kaustava Panda and Rukmini(2011) Screening of paint industry effluentfor heavy metal absorbing bacteria.International journal of Pharmaceutical Research. 2012, 4(3),74-76.ISSN No:0975-2366.
24.Praveen, Bisht, S.P.S and Ashok Kumar Panigrahy (2011) 16S rDNA sequencing ofantibiotic producing bacterial strain Lysinibacillus sp.(BP-3) from Nagavali river basin of Srikakulam, Andhra Pradesh,India, Indian Journal of Pharmaceutical education andresearch. 2012, Vol. 46, Issue 3, 217-221,Jul-Sep 2012. ISSN No:0019-5464.
25. B.Praveen Tripti sahu, amrita kumari panda, satpal s.Bisht andAshok kumar panigrahi(2016). Isolation and molecular characterization of few bacterial strains for their antimicrobial potential from nagavali river basin srikakulam, AndhraPradesh, India. The BioScan.11(2): 885-889, 2016.
26. B.Praveen,Ravishankar.L,Sagar.L,Saurav Barman.(2018).Biofertilizers-A noble technology for sustainable agriculture.- A Review. Asian Journal of Bioscience 13 (1) 50-53.
NCBI,GENBANK SUBMISSION:
1. Boddana,P., Bisht,S. and Panda,A.K. (2010). Pseudomonas sp. BP-1 16S ribosomal RNAgene, partial sequence. GenBank Accession No. HM359121.
2. Praveen,B., Bisht,S., Mishra,R.and Achary,B. (2010). Pseudomonas sp. BP-2 16S ribosomalRNA gene, partialsequence. GenBank AccessionNo. HM359122
3. Bisht,S., Boddana,P., Panda,K.,Behera, S.K. and Panda,A.K. (2010). Lysinibacillus sp. BP-316S ribosomal RNA gene,partial sequence. GenBank Accession No. HM359123
4. Bisht,S., Panda,A.K., Behera,S.K., Boddana,P. and Mishra,R. (2010).Acinetobacter sp. AK-P116S ribosomal RNA gene,partial sequence. GenBank Accession No. HM359120.
PI- Dr.B.Praveen
Co PI-Dr.Raghu Gogada
Biosurfactants, surface-active amphiphilic compounds, despite having a wide range of applications, have a high cost of production, which severely restricts their use. Bacteria from agro industrial wastes and marine sources possess the ability to produce lipopeptide biosurfactants that could have scientific and commercial benefit. For cheaper production of biosurfactant, we will be investigating the potential of the indigenously isolated biosurfactant producing organisms that grow on different cheap carbon sources (molasses, whey, and extracts of potato peels, orange peels, banana peels, and bagasse) through media optimization. The biosurfactants produced will be analyzed by Fourier Transform Infrared Spectroscopy (FT-IR). +AKA-Biosurfactant mediated synthesis of nanoparticles has now emerged as a green alternative for enhancing both nanoparticles synthesis +ACY- stabilization. . One of the modes of action is through adsorbing onto metallic nanoparticles, surface stabilizing the nanoparticles +ACY- preventing aggregation. Hence, biosurfactant mediated production of silver nanoparticles will be utilized in the present work. The primary characterization of the Ag nano particles will be done by visual observation, further, will be characterized using UV-Vis Spectrophotometer and TEM .Biosurfactant mediated Ag nano particles will be assessed for the antimicrobial studies on MDR strains of bacteria and fungi (S. aureus and Candida sp.).
PI- Dr.Raghu Gogada
Co PI- Dr.B.Praveen
Abstract:
This project is to investigate high affinity ABC metal transporters specific for Mn/Zn in Pseudomonas at the genetic level. Although P.
aeruginosa and P. putida were described as good potent metal accumulative bacteria, no study has ever been carried out to find the genes
responsible for these properties. The second point is the combinational use of two global analysis tools, bioinformatics, genomics and
proteomics. These compared total screens of Pseudomonas genomes based on evolutionary studies and on functional studies will strengthen
the confidence level of the future designated genes as being real metal transporters. The last point is the targeted approach. Once the metal
transporter genes will be selected, they will be specifically inactivated and their role in metal transporter efficiencies will be assessed precisely.
This is an advantage over random techniques of gene inactivation. The aim of the project is the identification and characterization of high
affinity ABC metal transporter genes from bacteria, with a focus on manganese and or zinc. These two metals are of particular interest because
they display a highly essential and are widespread in the environment due to their industrial applications. To face the bio-toxicity and
bioavailability of metals, bacteria have evolved transport systems, which allow them to grow in highly contaminated habitats. Identification,
isolation of Mn/Zn genes will permit to engineer bacteria and plants for bioremediation/fortification